stim1 cell signaling Search Results


stim1  (Cell Signaling Technology Inc)
95
Cell Signaling Technology Inc stim1
Primers for the target genes
Stim1, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/stim1/product/Cell Signaling Technology Inc
Average 95 stars, based on 1 article reviews
stim1 - by Bioz Stars, 2026-03
95/100 stars
  Buy from Supplier
rabbit anti stim1 antibody  (Cell Signaling Technology Inc)
95
Cell Signaling Technology Inc rabbit anti stim1 antibody
(A) Immunoblot analysis of cell fractions from 6-month-old WT and β-Gal − / − mice. Markers for the ER (Calnexin), PM (N-cadherin), and ER-PM junctions (ORAI1, <t>STIM1,</t> STIM2, VAPA, and VAPB) were enriched in their respective fractions. Immunoblots using HRP-conjugated cholera toxin B subunit (CTX-B) show high GM1 levels in β-Gal − / − fractions. (B) Representative HPTLC plate showing GM1 levels in the ER, PM, and ER-PM junctions isolated from 6-month-old WT and β-Gal − / − mice. STD, standard. Note: to detect GM1 in WT samples, the sample volume loaded was 3× that of the β-Gal − / − samples. (C) Quantification of GM1 levels from HPTLC plates performed in (B). n = 8. Values are expressed as median ± quartiles. Statistical analysis was performed using the Student’s t test; *** p < 0.001, **** p < 0.0001. (D) Representative HPTLC plate showing GM1 levels in ER-PM junctions isolated from 1-, 3-, and 6-month-old WT and β-Gal − / − mice. To detect GM1 in WT samples, the sample volume loaded was 3× that of the β-Gal − / − samples. (E) Quantification of GM1 levels from HPTLC plates performed in (D). n = 4. Values are expressed as median ± quartiles. Statistical analysis was performed using the Student’s t test with Welch’s correction; ns, not significant; *** p < 0.001, **** p < 0.0001.
Rabbit Anti Stim1 Antibody, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/rabbit anti stim1 antibody/product/Cell Signaling Technology Inc
Average 95 stars, based on 1 article reviews
rabbit anti stim1 antibody - by Bioz Stars, 2026-03
95/100 stars
  Buy from Supplier

Image Search Results


Primers for the target genes

Journal: Journal of Innate Immunity

Article Title: Mannan-Binding Lectin Reduces Epithelial-Mesenchymal Transition in Pulmonary Fibrosis via Inactivating the Store-Operated Calcium Entry Machinery

doi: 10.1159/000524693

Figure Lengend Snippet: Primers for the target genes

Article Snippet: The membranes were then stained with HRP-conjugated secondary antibody (S0001; Affinity Biosciences) at room temperature for another 1 h. Antibodies involved in this subsection are listed below: E-cadherin (14472, mouse anti-human monoclonal antibody, 1:1,000 dilution; Cell Signaling Technology), N-cadherin (22018-1-AP, rabbit anti-human polyclonal antibody, 1:1,000 dilution; Proteintech), α-SMA (14395-1-AP, rabbit anti-human polyclonal antibody, 1:1,000 dilution; Proteintech), vimentin (10366-1-AP, rabbit anti-human polyclonal antibody, 1:1,000 dilution; Proteintech), Orai1 (66223-1-Ig, rabbit anti-human polyclonal antibody, 1:1,000 dilution; Proteintech), Stim1 (5668, rabbit anti-human monoclonal antibody, 1:1,000 dilution; Cell Signaling Technology), SGK1 (23394-1-AP, rabbit anti-human polyclonal antibody, 1:1,000 dilution; Proteintech), PDK1 (17086-1-AP, rabbit anti-human polyclonal antibody, 1:1,000 dilution; Proteintech), GAPDH (60004-1-Ig, mouse anti-human monoclonal antibody, 1:1,000 dilution; Proteintech), and Ub (sc-8017, mouse anti-human monoclonal antibody, 1:1,000 dilution; Santa Cruz Biotechnology).

Techniques:

MBL mediated Orai1 ubiquitination. a, b HBE cells were incubated with 10-ng/mL TGF-β in the presence or absence of 10-μg/mL MBL for 24 h. a mRNA levels of Orai1 and Stim1 were assessed by quantitative RT-PCR. b Orai1 and Stim1 expression were determined by Western blot. c Orai1 and Stim1 levels in mice lung tissues were detected by Western blot. d HBE cells were incubated with 10-ng/mL TGF-β in the presence or absence of 10-μg/mL MBL for 24 h. Cells were treated with 10-μM MG132 4 h before being harvested. The ubiquitination level of Orai1 was detected by immunoprecipitation assay. ns, not significant, * p < 0.05, ** p < 0.01. The data represent three independent experiments with similar results. Unpaired Student's t test was used in a .

Journal: Journal of Innate Immunity

Article Title: Mannan-Binding Lectin Reduces Epithelial-Mesenchymal Transition in Pulmonary Fibrosis via Inactivating the Store-Operated Calcium Entry Machinery

doi: 10.1159/000524693

Figure Lengend Snippet: MBL mediated Orai1 ubiquitination. a, b HBE cells were incubated with 10-ng/mL TGF-β in the presence or absence of 10-μg/mL MBL for 24 h. a mRNA levels of Orai1 and Stim1 were assessed by quantitative RT-PCR. b Orai1 and Stim1 expression were determined by Western blot. c Orai1 and Stim1 levels in mice lung tissues were detected by Western blot. d HBE cells were incubated with 10-ng/mL TGF-β in the presence or absence of 10-μg/mL MBL for 24 h. Cells were treated with 10-μM MG132 4 h before being harvested. The ubiquitination level of Orai1 was detected by immunoprecipitation assay. ns, not significant, * p < 0.05, ** p < 0.01. The data represent three independent experiments with similar results. Unpaired Student's t test was used in a .

Article Snippet: The membranes were then stained with HRP-conjugated secondary antibody (S0001; Affinity Biosciences) at room temperature for another 1 h. Antibodies involved in this subsection are listed below: E-cadherin (14472, mouse anti-human monoclonal antibody, 1:1,000 dilution; Cell Signaling Technology), N-cadherin (22018-1-AP, rabbit anti-human polyclonal antibody, 1:1,000 dilution; Proteintech), α-SMA (14395-1-AP, rabbit anti-human polyclonal antibody, 1:1,000 dilution; Proteintech), vimentin (10366-1-AP, rabbit anti-human polyclonal antibody, 1:1,000 dilution; Proteintech), Orai1 (66223-1-Ig, rabbit anti-human polyclonal antibody, 1:1,000 dilution; Proteintech), Stim1 (5668, rabbit anti-human monoclonal antibody, 1:1,000 dilution; Cell Signaling Technology), SGK1 (23394-1-AP, rabbit anti-human polyclonal antibody, 1:1,000 dilution; Proteintech), PDK1 (17086-1-AP, rabbit anti-human polyclonal antibody, 1:1,000 dilution; Proteintech), GAPDH (60004-1-Ig, mouse anti-human monoclonal antibody, 1:1,000 dilution; Proteintech), and Ub (sc-8017, mouse anti-human monoclonal antibody, 1:1,000 dilution; Santa Cruz Biotechnology).

Techniques: Ubiquitin Proteomics, Incubation, Quantitative RT-PCR, Expressing, Western Blot, Immunoprecipitation

(A) Immunoblot analysis of cell fractions from 6-month-old WT and β-Gal − / − mice. Markers for the ER (Calnexin), PM (N-cadherin), and ER-PM junctions (ORAI1, STIM1, STIM2, VAPA, and VAPB) were enriched in their respective fractions. Immunoblots using HRP-conjugated cholera toxin B subunit (CTX-B) show high GM1 levels in β-Gal − / − fractions. (B) Representative HPTLC plate showing GM1 levels in the ER, PM, and ER-PM junctions isolated from 6-month-old WT and β-Gal − / − mice. STD, standard. Note: to detect GM1 in WT samples, the sample volume loaded was 3× that of the β-Gal − / − samples. (C) Quantification of GM1 levels from HPTLC plates performed in (B). n = 8. Values are expressed as median ± quartiles. Statistical analysis was performed using the Student’s t test; *** p < 0.001, **** p < 0.0001. (D) Representative HPTLC plate showing GM1 levels in ER-PM junctions isolated from 1-, 3-, and 6-month-old WT and β-Gal − / − mice. To detect GM1 in WT samples, the sample volume loaded was 3× that of the β-Gal − / − samples. (E) Quantification of GM1 levels from HPTLC plates performed in (D). n = 4. Values are expressed as median ± quartiles. Statistical analysis was performed using the Student’s t test with Welch’s correction; ns, not significant; *** p < 0.001, **** p < 0.0001.

Journal: Cell reports

Article Title: Altered GM1 catabolism affects NMDAR-mediated Ca 2+ signaling at ER-PM junctions and increases synaptic spine formation in a GM1-gangliosidosis model

doi: 10.1016/j.celrep.2024.114117

Figure Lengend Snippet: (A) Immunoblot analysis of cell fractions from 6-month-old WT and β-Gal − / − mice. Markers for the ER (Calnexin), PM (N-cadherin), and ER-PM junctions (ORAI1, STIM1, STIM2, VAPA, and VAPB) were enriched in their respective fractions. Immunoblots using HRP-conjugated cholera toxin B subunit (CTX-B) show high GM1 levels in β-Gal − / − fractions. (B) Representative HPTLC plate showing GM1 levels in the ER, PM, and ER-PM junctions isolated from 6-month-old WT and β-Gal − / − mice. STD, standard. Note: to detect GM1 in WT samples, the sample volume loaded was 3× that of the β-Gal − / − samples. (C) Quantification of GM1 levels from HPTLC plates performed in (B). n = 8. Values are expressed as median ± quartiles. Statistical analysis was performed using the Student’s t test; *** p < 0.001, **** p < 0.0001. (D) Representative HPTLC plate showing GM1 levels in ER-PM junctions isolated from 1-, 3-, and 6-month-old WT and β-Gal − / − mice. To detect GM1 in WT samples, the sample volume loaded was 3× that of the β-Gal − / − samples. (E) Quantification of GM1 levels from HPTLC plates performed in (D). n = 4. Values are expressed as median ± quartiles. Statistical analysis was performed using the Student’s t test with Welch’s correction; ns, not significant; *** p < 0.001, **** p < 0.0001.

Article Snippet: Rabbit anti-STIM1 antibody , Cell Signaling , Cat #: 4916; RRID: AB_2271287.

Techniques: Western Blot, High Performance Thin Layer Chromatography, Isolation

Journal: Cell reports

Article Title: Altered GM1 catabolism affects NMDAR-mediated Ca 2+ signaling at ER-PM junctions and increases synaptic spine formation in a GM1-gangliosidosis model

doi: 10.1016/j.celrep.2024.114117

Figure Lengend Snippet:

Article Snippet: Rabbit anti-STIM1 antibody , Cell Signaling , Cat #: 4916; RRID: AB_2271287.

Techniques: Virus, Recombinant, Isolation, Magnetic Beads, Plasmid Preparation, Software